This assessment issues the hot chromatographic technique – capillary electrochromatography(CEC) – that's lately receiving amazing realization. the rules of this technique basedon a mix of electroosmotic stream and analyte-stationary section interactions, CEC instrumentation,capillary column expertise, separation stipulations, and examples of a varietyof purposes are mentioned intimately.
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Extra info for Advances in Biochemical Engineering. Biotechnology Modern Adva
The very sensitive laser induced fluorescence is another option; however, only a small fraction of molecules fluoresce while a tedious functionalization must precede the detection of others. Mass spectrometry (MS) is likely to be the ideal choice for detection in CEC [165, 197–201]. The typical flow rates in CEC are in the range of 20–1000 nl/min and correspond well with those easily handled by nanoelectrospray ionization interfaces. Since MS is very sensitive, affords additional information about the molecular weight of separated compounds, and in MSn implementation allows even their identification, this truly orthogonal detection method appears most promising for the future.
82 1 Introduction The developments in molecular and cell biology in the last quarter of the 20th century led to new technologies for the production of complex biomolecules which have the potential to assist in human health care in the areas of diagnostics, prevention and treatment of diseases. One of the most important and at the same time most expensive step in their production is the isolation and cleaning (down-stream processing) of the target biomolecule(s). It represents more than 50% of the total costs of the production process.
As a result, the pressure drop along the column is one-third to one-fifth of that on columns packed with 3- or 5-mm beads. The resulting pressure drop is therefore much lower, allowing operation at higher flow rates while the optimized separation efficiencies are comparable to silica columns packed with 4 mm particles . Scaled up units intended for semi-preparative purposes were developed as well and were first reported by Schulte et al. . 014 mm and this allows even higher flow rates to be used then in case of the analytical ones.
Advances in Biochemical Engineering. Biotechnology Modern Adva